Properties and Regulation of Glutathione Peroxidase
نویسندگان
چکیده
منابع مشابه
Properties and regulation of glutathione peroxidase.
GSH peroxidase has been purified approximately ZOO-fold from pig’s blood. With cumene hydroperoxide as substrate, kinetic analysis on the purified enzyme gave nonlinear Lineweaver-Burk plots for the hydroperoxide substrate. Linear Lineweaver-Burk plots were obtained for GSH and a limiting K, value of approximately 3 mu was obtained for GSH. A limited analysis of the rate data has been carried o...
متن کاملThe purification and properties of glutathione peroxidase of erythrocytes.
Previous studies (1,2) have shown the presence of a peroxidase in erythrocytes which catalyzes the breakdown of hydrogen peroxide with reduced glutathione serving as a hydrogen donor. The significance of this enzyme in the protection of hemoglobin from oxidative breakdown has been shown previously (2). The erythrocyte reduced glutathione peroxidase has now been purified by column chromatography...
متن کاملPurification and properties of human erythrocyte glutathione peroxidase.
Glutathione peroxidase has been purified to homogeneity from human erythrocytes. The purification steps involved ammonium sulfate precipitation of hemolysate, CM-cellulose (CM-52), DEAE-cellulose (DE52), Sephadex G-200, and DEAE-Sephadex column chromatography. In the last step, i.e. DEAE-Sephadex A-25 column chromatography, the enzyme was eluted in a major peak and tailing fraction. The major p...
متن کاملPurification and properties of glutathione peroxidase from human placenta.
Glutathione peroxidase (glutathione--H2O2 oxidoreductase; EC 1.11.1.9) was purified to homogeneity from human placenta by using (NH4)2SO4 precipitation, ion-exchange chromatography, Sephadex gel filtration and preparative polyacrylamide-disc-gel electrophoresis. Glutathione peroxidase from human placenta is a tetramer, having 4g-atoms of selenium/mol of protein. The molecular weight of the enzy...
متن کاملsome properties of fuzzy hilbert spaces and norm of operators
in this thesis, at first we investigate the bounded inverse theorem on fuzzy normed linear spaces and study the set of all compact operators on these spaces. then we introduce the notions of fuzzy boundedness and investigate a new norm operators and the relationship between continuity and boundedness. and, we show that the space of all fuzzy bounded operators is complete. finally, we define...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62973-4